Hydroperoxides, how to find you?
Today, dedicated to the literature review on the different methods to quantify hydroperoxides, or more precisely, the activity of lipoxygenase (LOX). Many methods are available…funny how you start thinking that only the ones that you use are pertinent…Many others could be suitable…if only time to try them out was available!
Some methods are very easy and direct to use, such as the one at 234 nm but they require aqueous systems. Others call for the performance of extra enzymatic reactions before indirect measurement. Still more ask for expensive detectors or long and complicated experimental procedure to obtain the precious data.
From what I could read, spetrophotometic measurement of a color compound produced by the interaction of the products of the reaction and some reagents may prove the most suitable in terms of time required for analysis and sensitivity in organic solvent media. But again…many alternatives exist! Really, the task of writing a complete literature review on this subject is overwhelming…must one stop before satisfaction?!
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